Megagametophyte-derived linkage maps of white spruce ( Picea glauca) based on RAPD, SCAR and ESTP markers

I. Gosselin; Y. Zhou; J. Bousquet; N. Isabel

doi:10.1007/s00122-001-0823-y

Megagametophyte-derived linkage maps of white spruce ( Picea glauca) based on RAPD, SCAR and ESTP markers

Theor Appl Genet. 2002 May;104(6-7):987-997. doi: 10.1007/s00122-001-0823-y. Epub 2002 Feb 13.

Authors

I. Gosselin¹, Y. Zhou, J. Bousquet, N. Isabel

Affiliation

¹ Centre de recherche en biologie forestière and Chaire de recherche du Canada en génomique forestière et environnementale, Pavillon Charles-Eugène-Marchand, Université Laval, Ste-Foy Québec, Canada G1K 7P4.

PMID: 12582604
DOI: 10.1007/s00122-001-0823-y

Abstract

We have constructed linkage maps for two parents of white spruce [ Picea glauca (Moench) Voss]. Haploid megagametophytes from 92 and 96 seeds of parents M2 and 80132, respectively, were analysed with RAPD, SCAR and ESTP markers. Fragments segregating in a 1:1 Mendelian ratio were classified and mapped using MAPMAKER, GMENDEL and JOINMAP. For M2, the analysis with JOINMAP resulted in 165 loci (152 RAPDs, 3 SCARs and 10 ESTPs) mapping to 23 linkage groups and covering 2,059.4 cM(Kosambi function, K). For 80132, the analysis resulted in 144 loci (137 RAPDs, 1 SCAR and 7 ESTPs) mapping to 19 linkage groups and covering 2,007.7 cM(K). The maps covered 87 and 73% of the entire genome of parents M2 and 80132, respectively. Similar results were obtained with MAPMAKER and GMENDEL. A comparison was made between the two individual maps and 16 loci were shared between the two maps.