Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice

Cancer Lett. 2003 Mar 10;191(2):155-64. doi: 10.1016/s0304-3835(02)00677-8.

Abstract

Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Deletion
  • Chromosomes, Human, Pair 3 / genetics*
  • DNA Methylation
  • DNA Primers / chemistry
  • DNA, Neoplasm / metabolism
  • Down-Regulation*
  • Fibrosarcoma / genetics*
  • Fibrosarcoma / metabolism
  • Fibrosarcoma / pathology
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lactoferrin / genetics*
  • Lactoferrin / metabolism
  • Mice
  • Mice, SCID
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Neoplasm
  • Sarcoma, Experimental / genetics
  • Sarcoma, Experimental / metabolism
  • Sarcoma, Experimental / pathology
  • Transfection
  • Tumor Cells, Cultured

Substances

  • DNA Primers
  • DNA, Neoplasm
  • RNA, Neoplasm
  • Lactoferrin