Abstract
Two full-length cDNAs named CrRR2 and CrRR3 have been isolated by PCR from a C. roseus cDNA library. The first one encodes a 154 amino acid putative protein with a high percentage of identity with the Arabidopsis thaliana response regulators ARR16 and ARR17, and is not expressed in C. roseus organs and cell cultures. The second one encodes a 188 amino acid ORF sharing the highest homologies with the A. thaliana ARR8 and ARR9 response regulators. Its expression is root-specific and the transcripts are transiently up-regulated after trans-zeatin treatment in C. roseus suspension cells. CrRR3 protein might be involved in the cytokinin-enhanced alkaloid production in C. roseus cell cultures.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Catharanthus / cytology
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Catharanthus / genetics*
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Catharanthus / growth & development
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation, Developmental / drug effects
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Gene Expression Regulation, Plant / drug effects
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Molecular Sequence Data
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Plant Growth Regulators / genetics
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Plant Growth Regulators / metabolism
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Signal Transduction / genetics
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Signal Transduction / physiology
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Zeatin / pharmacology
Substances
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DNA, Complementary
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Plant Growth Regulators
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Plant Proteins
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Zeatin
Associated data
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GENBANK/AF534889
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GENBANK/AF534890