The effect of intraperitoneally administered recombinant IL-1beta on immune parameters and resistance to Aeromonas salmonicida in the rainbow trout (Oncorhynchus mykiss)

Dev Comp Immunol. 2003 Oct;27(9):801-12. doi: 10.1016/s0145-305x(03)00056-9.

Abstract

The present work provides the first information concerning the immunostimulatory activity of trout recombinant interleukin-1 beta (rIL-1beta) in vivo. The predicted rainbow trout mature IL-1beta peptide was produced as a recombinant protein in Escherichia coli. Optimal migration of peritoneal leucocytes and rIL-1beta induced phagocytosis occurred following intraperitoneal injection of 1 microg of the recombinant protein. Moreover, systemic IL-1beta, COX-2 and lysozyme II gene expression was enhanced following >or=1 microg rIL-1beta administration. Finally, resistance to Aeromonas salmonicida, the causative agent of furunculosis, was augmented at early times (2 days) post-injection of 1 microg rIL-1beta.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Aeromonas / immunology*
  • Animals
  • Cyclooxygenase 2
  • Dose-Response Relationship, Drug
  • Gram-Negative Bacterial Infections / immunology*
  • Immune System / drug effects*
  • Interleukin-1 / biosynthesis
  • Interleukin-1 / genetics
  • Interleukin-1 / pharmacology*
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Leukocyte Count
  • Leukocytes / drug effects
  • Muramidase / biosynthesis
  • Muramidase / genetics
  • Oncorhynchus mykiss / immunology*
  • Oncorhynchus mykiss / microbiology
  • Phagocytosis / drug effects
  • Prostaglandin-Endoperoxide Synthases / biosynthesis
  • Prostaglandin-Endoperoxide Synthases / genetics

Substances

  • Adjuvants, Immunologic
  • Interleukin-1
  • Isoenzymes
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Muramidase