Objective: To prepare DNA microarray for detecting both hepatitis B and D virus as (HBV and HDV).
Methods: With the assistance of Oligo6.4 software, specific PCR primers targeting the conserved region of HBV and HDV were designed. The PCR products were purified and cloned into the pMD18-T vectors, followed by rapid identification. The recombinant plasmids were then extracted from positive clones and the target gene fragments underwent sequence analysis.
Results: The gene fragments of HBV and HDV were obtained by PCR, which were confirmed by sequence analysis to be specific gene fragments of HBV and HDV.
Conclusion: Using PCR amplification products to prepare the DNA microarray is quick, simple and effective.