[Cloning, expression and purification of neural specific HuD cDNA]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2002 Jun;24(3):250-3.
[Article in Chinese]

Abstract

Objective: To prokaryoticly express and purify HuD protein and its RNA recognition motifs.

Methods: HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.

Results: Purified HuD protein and its RNA recognized motifs were observed.

Conclusions: The result might aid for basic research and clinical application.

Publication types

  • English Abstract

MeSH terms

  • Antibodies, Antinuclear / biosynthesis
  • Antibodies, Antinuclear / genetics
  • Antibodies, Antinuclear / isolation & purification
  • Carcinoma, Small Cell / genetics
  • Carcinoma, Small Cell / immunology
  • Carcinoma, Small Cell / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • ELAV Proteins
  • ELAV-Like Protein 4
  • Humans
  • Lung Neoplasms / genetics
  • Lung Neoplasms / immunology
  • Lung Neoplasms / metabolism
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / isolation & purification
  • Neurons / immunology
  • Paraneoplastic Syndromes, Nervous System / genetics*
  • Paraneoplastic Syndromes, Nervous System / immunology
  • Paraneoplastic Syndromes, Nervous System / metabolism
  • RNA-Binding Proteins / biosynthesis
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / isolation & purification

Substances

  • Antibodies, Antinuclear
  • DNA, Complementary
  • ELAV Proteins
  • ELAV-Like Protein 4
  • ELAVL4 protein, human
  • Nerve Tissue Proteins
  • RNA-Binding Proteins