Molecular evidence for transferrin receptor 2 expression in all FAB subtypes of acute myeloid leukemia

Leuk Res. 2003 Dec;27(12):1101-3. doi: 10.1016/s0145-2126(03)00100-0.

Abstract

We examined transferrin receptor (TfR) 1 and TfR2 mRNA expression in 50 acute myeloid leukemia (AML) patients by RT-PCR, with primers specific for exons 15-17 (TfR1), 3-5 (TfR2-alpha) and 4-5 (TfR2-beta) of the corresponding gene. There were 4/50 TfR1-negative (-), 3/50 TfR2-alpha mRNA (-) and 13/50 TfR2-beta mRNA (-) cases; only three cases were TfR1/2 mRNA (-). No significant correlations were identified between TfR2-beta mRNA negativity and specific FAB subtypes, karyotype or attainment of complete remission. These findings suggest that: (i) TfR2 expression is not restricted to erythroid cells, and (ii) iron import proteins might complement each other in AML cells.

Publication types

  • Comparative Study

MeSH terms

  • Acute Disease
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • DNA Primers / chemistry
  • Female
  • Humans
  • Immunoglobulin Fab Fragments / genetics*
  • Iron / metabolism
  • Leukemia, Myeloid / genetics*
  • Leukemia, Myeloid / immunology
  • Male
  • Middle Aged
  • RNA, Messenger / metabolism
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Receptors, Transferrin / genetics*
  • Remission Induction
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA Primers
  • Immunoglobulin Fab Fragments
  • RNA, Messenger
  • RNA, Neoplasm
  • Receptors, Transferrin
  • TFR2 protein, human
  • Iron