Abstract
FkpA is a peptidylprolyl isomerase whose expression is regulated by the alternative sigma factor, sigma factor E (sigma(E)). In contrast to the results of a previous report, inactivation of fkpA was found to have only a minor effect on the ability of Salmonella enterica serovar Typhimurium to invade and survive within epithelial and macrophage cell lines and cause infection in mice. However, an effect of the fkpA mutation on serovar Typhimurium virulence was seen if the mutation was combined with mutations in surA or htrA, two other sigma(E)-regulated genes, which encode proteins involved in protein folding and/or degradation in the periplasm.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Caco-2 Cells
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Carrier Proteins*
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Cell Line
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Genes, Bacterial
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Heat-Shock Proteins / physiology
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Humans
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Immunophilins / genetics
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Immunophilins / physiology*
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Mice
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Mutation
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Peptidylprolyl Isomerase / genetics
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Peptidylprolyl Isomerase / physiology*
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Periplasmic Proteins / physiology
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Salmonella typhimurium / enzymology*
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Salmonella typhimurium / genetics
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Salmonella typhimurium / pathogenicity*
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Serine Endopeptidases / physiology
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Sigma Factor / physiology
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Transcription Factors / physiology
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Virulence / genetics
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Virulence / physiology
Substances
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Carrier Proteins
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Heat-Shock Proteins
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Membrane Proteins
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Periplasmic Proteins
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Sigma Factor
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Transcription Factors
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sporulation-specific sigma factors
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DegP protease
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Serine Endopeptidases
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periplasmic peptidylprolyl cis-trans isomerase
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Immunophilins
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Peptidylprolyl Isomerase