Six compounds were isolated from the aqueous extract of Aconitum carmichaeli Debx (cultivated in Jiang-you region of Sichuan province). Five of them have been identified as uracil (I), songorine HCl(II), karakoline(III), neoline(IV), and fuzitine(VI). Compound V is a new C19-diterpenoid alkaloid determined as C33H47NO9 and named neojiangyouaconitine.