A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification

X de Lamballerie; C Zandotti; C Vignoli; C Bollet; P de Micco

doi:10.1016/0923-2508(92)90107-y

A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification

Res Microbiol. 1992 Oct;143(8):785-90. doi: 10.1016/0923-2508(92)90107-y.

Authors

X de Lamballerie¹, C Zandotti, C Vignoli, C Bollet, P de Micco

Affiliation

¹ Laboratoire de Microbiologie et d'Hygiène Hospitalières, Hôpital Salvator, Marseille, France.

PMID: 1298031
DOI: 10.1016/0923-2508(92)90107-y

Abstract

"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.

MeSH terms

Chelating Agents*
DNA, Bacterial / isolation & purification*
Gene Amplification
Gram-Negative Bacteria / genetics*
Gram-Positive Bacteria / genetics*
In Vitro Techniques
Polymerase Chain Reaction / methods*
Resins, Synthetic
Streptococcus agalactiae / genetics

Substances

Chelating Agents
DNA, Bacterial
Resins, Synthetic
Chelex 100