Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies

F Tozzini; D Matteucci; P Bandecchi; F Baldinotti; A Poli; M Pistello; K H Siebelink; L Ceccherini-Nelli; M Bendinelli

doi:10.1016/0166-0934(92)90026-a

Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies

J Virol Methods. 1992 Jun;37(3):241-52. doi: 10.1016/0166-0934(92)90026-a.

Authors

F Tozzini¹, D Matteucci, P Bandecchi, F Baldinotti, A Poli, M Pistello, K H Siebelink, L Ceccherini-Nelli, M Bendinelli

Affiliation

¹ Department of Animal Pathology, University of Pisa, Italy.

PMID: 1321834
DOI: 10.1016/0166-0934(92)90026-a

Abstract

The feline immunodeficiency virus (FIV) readily produced syncytia in Crandell feline kidney (CrFK) cells adapted to a medium containing 0.5% fetal calf serum, a variety of growth factors and other supplements. This finding has been exploited to develop simple and sensitive virus titration and neutralization assays. High titre neutralizing antibodies were detected in cats infected naturally and experimentally with FIV, but not in uninfected animals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Viral / blood*
Blotting, Western
Cats
Cell Line
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Feline Acquired Immunodeficiency Syndrome / diagnosis
Giant Cells / microbiology*
Immunodeficiency Virus, Feline / immunology*
Immunodeficiency Virus, Feline / isolation & purification*
Immunodeficiency Virus, Feline / physiology
Kidney / cytology
Neutralization Tests
Sensitivity and Specificity

Substances

Antibodies, Viral