Crystallization and preliminary X-ray analysis of three serotypes of foot-and-mouth disease virus

J Mol Biol. 1992 Dec 20;228(4):1263-8. doi: 10.1016/0022-2836(92)90332-e.

Abstract

Foot-and-mouth disease viruses from serotypes O, A and C have been crystallized. The particular strains studied include O1K, A10(61), A22 Iraq 24/64, A24 Cruzeiro and C-S8c1. In addition, crystals have been grown of G67, a monoclonal antibody neutralization escape mutant derived from O1K, and of virus R100, recovered after the establishment of a persistent infection in baby hamster kidney cells with C-S8c1. Empty particles, capsids which lack the RNA genome, have also been crystallized for subtypes A22 Iraq 24/64 and A10(61). In almost all cases, crystals suitable for high resolution structure determination were obtained from (NH4)2SO4 or mixtures of polyethylene glycol and NH4Cl.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aphthovirus / chemistry*
  • Aphthovirus / genetics
  • Aphthovirus / ultrastructure
  • Capsid / chemistry
  • Capsid / ultrastructure
  • Crystallization
  • Genetic Variation
  • Mutation
  • Serotyping
  • Trypsin / pharmacology
  • Virion / chemistry
  • Virion / drug effects
  • Virion / genetics
  • Virion / ultrastructure
  • X-Ray Diffraction

Substances

  • Trypsin