Amplification of reproducible allele markers for amplified fragment length polymorphism analysis

Biotechniques. 1992 Jan;12(1):16, 18, 20-2.

Abstract

A procedure for amplification by PCR of reproducible allele markers for amplified fragment length polymorphism (Amp-FLP) analysis is presented. We have prepared markers for the allelic products of the VNTR loci D1S80 (MCT118) and D17S30 (YNZ22) and for the hypervariable VNTR locus close to the 3' end of the apolipoprotein B gene (apoB) by re-amplifying a mixture of PCR products from individuals with known alleles. These allele markers allow precise and discrete determination of the VNTR alleles at these loci using the Amp-FLP technique that should prove suitable in forensic analyses, paternity testing and population studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Apolipoproteins B / genetics*
  • Base Sequence
  • DNA / chemistry
  • DNA / genetics*
  • Forensic Medicine
  • Genetic Markers*
  • Humans
  • Male
  • Molecular Sequence Data
  • Paternity
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*
  • Repetitive Sequences, Nucleic Acid

Substances

  • Apolipoproteins B
  • Genetic Markers
  • DNA