Benarthin: a new inhibitor of pyroglutamyl peptidase. I. Taxonomy, fermentation, isolation and biological activities

T Aoyagi; M Hatsu; F Kojima; C Hayashi; M Hamada; T Takeuchi

doi:10.7164/antibiotics.45.1079

Benarthin: a new inhibitor of pyroglutamyl peptidase. I. Taxonomy, fermentation, isolation and biological activities

J Antibiot (Tokyo). 1992 Jul;45(7):1079-83. doi: 10.7164/antibiotics.45.1079.

Authors

T Aoyagi¹, M Hatsu, F Kojima, C Hayashi, M Hamada, T Takeuchi

Affiliation

¹ Institute of Microbial Chemistry, Tokyo, Japan.

PMID: 1355471
DOI: 10.7164/antibiotics.45.1079

Abstract

We found benarthin, a new inhibitor of pyroglutamyl peptidase, in the fermentation broth of Streptomyces xanthophaeus MJ244-SF1. It was purified by column chromatography and centrifugal partition chromatography (CPC) and then was isolated as a colorless powder. The binding of benarthin was competitive with substrate and its inhibition constant (Ki) was 1.2 x 10(-6) M.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Classification
Dipeptides / isolation & purification*
Dipeptides / pharmacology
Fermentation
Pyroglutamyl-Peptidase I / antagonists & inhibitors
Streptomyces / classification*

Substances

Dipeptides
benarthin
Pyroglutamyl-Peptidase I