Characterization of the endogenous reverse transcriptase reaction products of SIVagmTYO-7, a simian immunodeficiency virus isolated from an African green monkey

Virus Res. 1992 Feb;22(2):143-9. doi: 10.1016/0168-1702(92)90040-g.

Abstract

The products of the endogenous reverse transcriptase reaction of SIVagmTYO-7 were characterized after the reaction conditions had been optimized. The major reaction product in the presence of actinomycin D and oligo(dT) was a DNA with a size of 300 bases. Without actinomycin D two additional reaction products with 600 or 700 bases appeared. The 300 base product was identified as the (-)strong-stop DNA, whereas the 700 base product is the (+)strong-stop DNA. The 600 base product appeared only after oligo(dT) priming. The (-)strong-stop DNA hybridized specifically with a 9 kb RNA found in virus particles and three RNA species of 1.8, 4.8 and 9 kb isolated from SIVagmTYO-7 infected cells.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Line
  • Chlorocebus aethiops
  • Oligonucleotide Probes
  • RNA, Viral / chemistry
  • RNA, Viral / isolation & purification
  • RNA-Directed DNA Polymerase / chemistry
  • RNA-Directed DNA Polymerase / genetics
  • RNA-Directed DNA Polymerase / isolation & purification*
  • Simian Immunodeficiency Virus / enzymology*
  • Simian Immunodeficiency Virus / genetics
  • Simian Immunodeficiency Virus / isolation & purification

Substances

  • Oligonucleotide Probes
  • RNA, Viral
  • RNA-Directed DNA Polymerase