Three additional inositol 1,4,5-trisphosphate receptors: molecular cloning and differential localization in brain and peripheral tissues

Proc Natl Acad Sci U S A. 1992 May 15;89(10):4265-9. doi: 10.1073/pnas.89.10.4265.

Abstract

Three inositol 1,4,5-trisphosphate receptor (IP3R) cDNAs, designated IP3R-II, -III, and -IV, were cloned from a mouse placenta cDNA library. All three display strong homology in membrane-spanning domains M7 and M8 to the originally cloned cerebellar IP3R-I, with divergences predominantly in cytoplasmic domains. Levels of mRNA for the three additional IP3Rs in general are substantially lower than for IP3R-I, though in the gastrointestinal tract the levels of IP3R-III may be comparable to IP3R-I. Cerebellar Purkinje cells express at least two and possibly three distinct IP3Rs, suggesting heterogeneity of IP3 action within a single cell.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain / physiology*
  • Calcium Channels*
  • Cell Membrane / metabolism
  • DNA / genetics
  • DNA / isolation & purification
  • Female
  • Genetic Variation*
  • Gestational Age
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Inositol 1,4,5-Trisphosphate Receptors
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodeoxyribonucleotides
  • Organ Specificity
  • Placenta / physiology
  • Pregnancy
  • RNA / genetics
  • RNA / isolation & purification
  • Receptors, Cell Surface / genetics*
  • Receptors, Cytoplasmic and Nuclear*
  • Sequence Homology, Nucleic Acid

Substances

  • Calcium Channels
  • Inositol 1,4,5-Trisphosphate Receptors
  • Oligodeoxyribonucleotides
  • Receptors, Cell Surface
  • Receptors, Cytoplasmic and Nuclear
  • RNA
  • Inositol 1,4,5-Trisphosphate
  • DNA

Associated data

  • GENBANK/M90087
  • GENBANK/M90088
  • GENBANK/M90089