Release of sensory CGRP by hypertonic NaCl is not blocked by tetrodotoxin, omega-conotoxin, nifedipine and ruthenium red

E Del Bianco; M Tramontana; C Bertrand; P Geppetti

doi:10.1016/0024-3205(92)90234-g

Release of sensory CGRP by hypertonic NaCl is not blocked by tetrodotoxin, omega-conotoxin, nifedipine and ruthenium red

Life Sci. 1992;51(8):PL73-6. doi: 10.1016/0024-3205(92)90234-g.

Authors

E Del Bianco¹, M Tramontana, C Bertrand, P Geppetti

Affiliation

¹ Institute of Internal Medicine IV, University of Florence, Italy.

PMID: 1379316
DOI: 10.1016/0024-3205(92)90234-g

Abstract

Hypertonic NaCl (160 mM added to the physiological salt solution) releases CGRP in a Ca(2+)-dependent manner from capsaicin-sensitive sensory nerves of the rat urinary bladder. The NaCl (160 mM)-evoked CGRP release was not affected by tetrodotoxin (0.3 microM), nifedipine (1 microM), omega-conotoxin (0.1 microM) and ruthenium red (10 microM). NaCl (160 mM)-evokes release of sensory neuropeptides without the involvement of axon reflexes, and by promoting Ca2+ influx via a dihydropyridine omega-conotoxin and ruthenium red insensitive pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcitonin Gene-Related Peptide / drug effects*
Calcitonin Gene-Related Peptide / metabolism
Calcium Channel Blockers / pharmacology*
In Vitro Techniques
Male
Nifedipine / pharmacology
Peptides, Cyclic / pharmacology
Rats
Rats, Inbred Strains
Ruthenium Red / pharmacology
Saline Solution, Hypertonic / pharmacology*
Tetrodotoxin / pharmacology*
omega-Conotoxins*

Substances

Calcium Channel Blockers
Peptides, Cyclic
Saline Solution, Hypertonic
omega-Conotoxins
Conus magus toxin
Ruthenium Red
Tetrodotoxin
Nifedipine
Calcitonin Gene-Related Peptide