T cell receptor V beta repertoire of double-negative alpha/beta T cells in patients with systemic sclerosis

Arthritis Rheum. 1992 Aug;35(8):944-8. doi: 10.1002/art.1780350815.

Abstract

Objective: To analyze the T cell receptor V beta gene on double-negative (DN) alpha/beta T cells, which are increased in number, on peripheral blood lymphocytes (PBL) from patients with systemic sclerosis (SSc).

Methods: The DN alpha/beta T cells were sorted by flow cytometry from PBL obtained from 3 patients with SSc. The V beta repertoire was analyzed by polymerase chain reaction.

Results: Only 1 or 2 V beta genes (V beta 5/7, 5, or 17) were predominantly expressed on DN alpha/beta T cells from these 3 patients.

Conclusion: The V beta repertoire on DN alpha/beta T cells in PBL from patients with SSc is rather restricted.

MeSH terms

  • Base Sequence
  • DNA / analysis
  • DNA / genetics
  • Flow Cytometry
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA / analysis
  • RNA / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Scleroderma, Systemic / genetics*
  • Scleroderma, Systemic / pathology
  • T-Lymphocytes / chemistry
  • T-Lymphocytes / pathology*
  • T-Lymphocytes / ultrastructure

Substances

  • Receptors, Antigen, T-Cell, alpha-beta
  • RNA
  • DNA