Biotin-hydrazide derivatives for the development of steroid enzyme-linked immunoassays

J Steroid Biochem Mol Biol. 1992 Nov;43(6):523-7. doi: 10.1016/0960-0760(92)90239-f.

Abstract

We are describing the synthesis and use of biotinamidocaproyl-derivatives of 18-oxocortisol-3-carboxymethoxylamine for the development of enzyme labels using the avidin-peroxidase system for the design of enzyme-linked immunoassays (ELISA). An ELISA for 18-oxocortisol and -hydroxycorticosterone was devised which showed improved sensitivity and specificity in comparison to an RIA using a tritiated tracer. The system is easy to prepare and offers the possibility to design immunoassays when no tritiated tracer is available.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 18-Hydroxycorticosterone / analogs & derivatives
  • 18-Hydroxycorticosterone / analysis*
  • 18-Hydroxycorticosterone / immunology
  • Binding, Competitive
  • Biotin / analogs & derivatives*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Hydrazines
  • Hydrocortisone / analogs & derivatives*
  • Hydrocortisone / analysis
  • Hydrocortisone / immunology

Substances

  • Hydrazines
  • 18-oxocortisol
  • hydrazine
  • 18-Hydroxycorticosterone
  • Biotin
  • Hydrocortisone