Two-dimensional electrophoresis of cat sera: protein identification by cross reacting antibodies against human serum proteins

Electrophoresis. 1992 Jul;13(7):450-3. doi: 10.1002/elps.1150130193.

Abstract

After two-dimensional electrophoresis of cat serum, individual proteins were identified by means of cross reacting antibodies against the human protein homologues. Testing by cross reactivities seemed the method of choice because immunoreagents for cat proteins are not easily available.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology*
  • Antibody Specificity
  • Blood Proteins / analysis*
  • Cats / blood*
  • Electrophoresis, Gel, Two-Dimensional*
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunoblotting
  • Isoelectric Focusing
  • Isoelectric Point
  • Species Specificity

Substances

  • Antibodies
  • Blood Proteins