Detection of simian immunodeficiency virus DNA in macrophages from infected rhesus macaques

J Med Primatol. 1992 Feb-May;21(2-3):74-81.

Abstract

We have examined the frequency of infection of monocyte-derived and alveolar macrophages isolated from rhesus macaques inoculated with simian immunodeficiency virus (SIVmac) utilizing a semiquantitative PCR methodology. Animals were inoculated with either pathogenic (SIVmac239) or nonpathogenic (SIVmac1A11) molecularly cloned viruses of SIVmac, or with uncloned pathogenic SIVmacBIOL. The frequency of SIV DNA in macrophages was highest early after infection and at terminal stages of disease, whereas during the asymptomatic period, SIV DNA was present at very low levels in macrophages.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Bronchoalveolar Lavage Fluid / cytology
  • Cells, Cultured
  • DNA, Viral / analysis*
  • DNA, Viral / chemistry
  • Macaca mulatta*
  • Macrophages / microbiology*
  • Macrophages, Alveolar / microbiology
  • Molecular Sequence Data
  • Monocytes / microbiology
  • Polymerase Chain Reaction
  • Simian Acquired Immunodeficiency Syndrome / microbiology*
  • Simian Immunodeficiency Virus / genetics
  • Simian Immunodeficiency Virus / isolation & purification*

Substances

  • DNA, Viral