Abstract
Priming of human polymorphonuclear granulocytes (PMNs) with cytokines (IL-3, IL-6, TNF-alpha) followed by a subsequent stimulation (FMLP) led to an enhanced polymerization of actin and GTPase-activity which correlated to a loss of ras immunoreactivity and an increased expression of Rab proteins. Furthermore TNF-alpha and 12-HETE induced the heat shock proteins (hsp 70 family) in PMNs as was demonstrated by metabolic radiolabeling and Western blotting (anti-hsp 72). This activation of the stress response exerted a protective function towards a subsequent lytic attack by a bacterial cytolysin (leukocidin).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
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Actins / metabolism
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Blotting, Western
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Cytokines / pharmacology*
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Eicosanoids / pharmacology*
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GTP Phosphohydrolases / metabolism
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Heat-Shock Proteins / biosynthesis*
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Humans
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Hydroxyeicosatetraenoic Acids / pharmacology
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Interleukin-3 / pharmacology
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Interleukin-6 / pharmacology
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Neutrophils / metabolism*
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Tumor Necrosis Factor-alpha / pharmacology
Substances
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Actins
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Cytokines
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Eicosanoids
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Heat-Shock Proteins
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Hydroxyeicosatetraenoic Acids
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Interleukin-3
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Interleukin-6
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Tumor Necrosis Factor-alpha
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N-Formylmethionine Leucyl-Phenylalanine
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
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GTP Phosphohydrolases