Abstract
Tfb4 is identified as a subunit of the core complex of yeast RNA polymerase II general transcription factor IIH (TFIIH) by affinity purification, by peptide sequence analysis, and by expression of the entire complex in insect cells. Tfb3, previously identified as a component of the core complex, is shown instead to form a complex with cdk and cyclin subunits of TFIIH. This reassignment of subunits resolves a longstanding discrepancy between yeast and human TFIIH complexes.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Baculoviridae / genetics
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Cyclin-Dependent Kinases / chemistry
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Cyclins / chemistry
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Electrophoresis, Polyacrylamide Gel
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Gene Expression
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Genetic Vectors
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Glutathione Transferase / genetics
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Subunits / chemistry*
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae
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Saccharomyces cerevisiae Proteins*
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Spodoptera / metabolism
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TATA-Binding Protein Associated Factors*
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Transcription Factor TFIID*
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Transcription Factor TFIIH
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Transcription Factors, TFII / chemistry*
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Transcription Factors, TFII / genetics
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Transcription Factors, TFII / isolation & purification
Substances
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Cyclins
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Protein Subunits
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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TAF6 protein, S cerevisiae
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TATA-Binding Protein Associated Factors
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Transcription Factor TFIID
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Transcription Factors, TFII
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Transcription Factor TFIIH
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Glutathione Transferase
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Cyclin-Dependent Kinases