Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues

Vet Immunol Immunopathol. 2003 Nov 15;96(1-2):65-72. doi: 10.1016/s0165-2427(03)00158-2.

Abstract

This study evaluates the cross-reactivity of seven anti-human and one anti-bovine antibodies in formalin-fixed, paraffin-embedded tissue samples of liver and mesenteric lymph nodes of 13 striped dolphins (Stenella coeruleoalba). Four antibodies (CD3, IgG, lysozyme and S100 protein) reacted with striped dolphin lymph nodes in a similar pattern to that observed in the species of origin. The anti-human MHC class II mAb reacted strongly with macrophages and dendritic-like cells of striped dolphins, whereas a small number of lymphocytes were labelled with this antibody. These antibodies were used to study the immunophenotype of the inflammatory infiltrated in non-specific chronic reactive hepatitis (eight cases) and chronic parasite cholangitis (two cases) and normal liver (three cases) of striped dolphins. Non-specific chronic reactive hepatitis was composed of inflammatory infiltration of CD3+ T lymphocytes and IgG+ plasma cells in portal spaces and hepatic sinusoids. Lymphonodular aggregates observed in chronic parasitic cholangitis showed a cellular distribution similar to that found in lymph node cortex, including the presence of S100+ and MHC class II+ dendritic-like cells in lymphoid follicles and interfollicular areas. This result suggests that those inflammatory infiltrates are highly organised to enhance antigen presentation to B and T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • CD3 Complex / immunology
  • Cattle
  • Cross Reactions
  • Dolphins / immunology*
  • Female
  • Hepatitis, Animal / immunology
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Immunoglobulin G / immunology
  • Immunohistochemistry / veterinary
  • Liver / immunology*
  • Lymph Nodes / immunology*
  • Male
  • Muramidase / immunology
  • Paraffin Embedding / veterinary
  • S100 Proteins / immunology

Substances

  • Antibodies, Monoclonal
  • CD3 Complex
  • Histocompatibility Antigens Class II
  • Immunoglobulin G
  • S100 Proteins
  • Muramidase