Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species

F Kontos; E Petinaki; I Spiliopoulou; M Maniati; A N Maniatis

doi:10.1016/s0167-7012(03)00173-8

Evaluation of a novel method based on PCR Restriction Fragment Length Polymorphism Analysis of the tuf gene for the identification of Staphylococcus species

J Microbiol Methods. 2003 Nov;55(2):465-9. doi: 10.1016/s0167-7012(03)00173-8.

Authors

F Kontos¹, E Petinaki, I Spiliopoulou, M Maniati, A N Maniatis

Affiliation

¹ Department of Microbiology, School of Medicine, University of Thessalia, Papakyriazi 22, Larissa, Greece.

PMID: 14529969
DOI: 10.1016/s0167-7012(03)00173-8

Abstract

A novel method, based on PCR Restriction Fragment Length Polymorphism Analysis (PRA) of a part of the tuf gene (370 bp), was designed for the identification of 11 staphylococcal species, including the most common staphylococcal pathogens. A total of 258 clinical isolates were validated by this assay, and the results were in concordance with those obtained by the reference method of Kloos and Schleifer.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Algorithms
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Humans
Peptide Elongation Factor Tu / chemistry
Peptide Elongation Factor Tu / genetics*
Polymerase Chain Reaction / methods*
Polymorphism, Restriction Fragment Length*
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus / classification
Staphylococcus / genetics*

Substances

DNA, Bacterial
Peptide Elongation Factor Tu