Abstract
The double-stranded RNA-activated protein kinase-binding domain (PKRbd) of the NS5A gene of hepatitis C virus (HCV) was studied by the cloning and sequencing method, in HCV-infected patients who had a primary resistance to treatment with interferon (IFN)-alpha (early nonresponders). Patients whose virus load decreased by >or=0.5 log (early responders) were similarly analyzed. In the 2 groups, the PKRbd evolved similarly over the first 24 h. Selection of resistant HCV variants is unlikely to explain primary resistance to IFN-alpha.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antiviral Agents / blood
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Antiviral Agents / pharmacokinetics
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Antiviral Agents / therapeutic use*
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Area Under Curve
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Genetic Variation
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Hepacivirus / genetics
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Hepacivirus / immunology*
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Hepatitis C / drug therapy
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Hepatitis C / immunology*
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Humans
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Interferon-alpha / blood
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Interferon-alpha / pharmacokinetics
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Interferon-alpha / therapeutic use*
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RNA, Viral / chemistry
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RNA, Viral / genetics
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Statistics, Nonparametric
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Viral Load
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Viral Nonstructural Proteins / genetics*
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Viral Nonstructural Proteins / immunology
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Virus Replication / genetics
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Virus Replication / immunology
Substances
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Antiviral Agents
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Interferon-alpha
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RNA, Viral
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Viral Nonstructural Proteins
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NS-5 protein, hepatitis C virus