[Human herpesvirus 6 infections in kidney and bone marrow/stem cell transplant recipients]

Mikrobiyol Bul. 2003 Apr-Jun;37(2-3):179-86.
[Article in Turkish]

Abstract

In this study, active human herpesvirus (HHV)-6 infection were investigated in 39 renal and 9 bone marrow/stem cell transplant recipients. For this purpose, the presence of HHV-6 DNA in patients sera have been searched by nested polymerase chain reaction (nPCR). In addition, HHV-6 IgM and IgG antibodies were performed by micro-enzyme immunoassay (EIA) to detect seronegative patients before transplantation and IgM response in active or primary HHV-6 infection. Active infection with HHV-6 DNA positivity was detected in 5.3% of renal and 22.2% of bone marrow/stem cell transplant recipients. Active HHV-6 infection was found to be related with asymptomatic reactivation, graft disfunction and cytomegalovirus disease in renal transplant recipients, and, fever and graft versus host disease in bone marrow/stem cell transplant recipients. It has been concluded that, the investigation of HHV-6 DNA by nPCR in the transplant sera, was a practical and useful method for the laboratories, in order to diagnose active HHV-6 infection, while HHV-6 IgG antibody detection was also useful for the differential diagnosis of primary infection or reactivation/reinfection, but HHV-6 IgM antibodies has low value to detect active HHV-6 infection.

Publication types

  • English Abstract

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Viral / blood
  • Bone Marrow Transplantation* / immunology
  • Child
  • Child, Preschool
  • DNA, Viral / blood
  • Diagnosis, Differential
  • Female
  • Herpesvirus 6, Human / genetics
  • Herpesvirus 6, Human / immunology
  • Herpesvirus 6, Human / isolation & purification*
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Infant
  • Kidney Transplantation* / immunology
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Recurrence
  • Roseolovirus Infections / diagnosis
  • Roseolovirus Infections / etiology*

Substances

  • Antibodies, Viral
  • DNA, Viral
  • Immunoglobulin G
  • Immunoglobulin M