Abstract
A PCR-enzyme-linked immunosorbent assay and a real-time PCR assay were compared for diagnosis and follow-up of cerebral toxoplasmosis in a stem cell transplant recipient. The sensitivity of detection was similar for both assays but was higher when the assays were performed on buffy coat rather than on whole blood or serum.
Publication types
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Case Reports
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Comparative Study
MeSH terms
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Adult
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Animals
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Antibodies, Protozoan / blood
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Enzyme-Linked Immunosorbent Assay / methods
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Humans
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Immunoglobulin G / blood
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / therapy
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Male
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Polymerase Chain Reaction / methods*
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Stem Cell Transplantation / adverse effects*
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Toxoplasma / classification
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Toxoplasma / isolation & purification*
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Toxoplasmosis, Cerebral / blood
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Toxoplasmosis, Cerebral / diagnosis*
Substances
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Antibodies, Protozoan
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Immunoglobulin G