Molecular diagnosis of a vascular prosthesis infection, due to Propionibacterium acnes, by amplification and sequencing of 16S rDNA

L Le Page; I Podglajen; E Chemla; J L Mainardi

doi:10.1046/j.1469-0691.2003.00764.x

Molecular diagnosis of a vascular prosthesis infection, due to Propionibacterium acnes, by amplification and sequencing of 16S rDNA

Clin Microbiol Infect. 2003 Nov;9(11):1125-7. doi: 10.1046/j.1469-0691.2003.00764.x.

Authors

L Le Page¹, I Podglajen, E Chemla, J L Mainardi

Affiliation

¹ Unité Mobile de Microbiologie Clinique, Clinical Microbiology, Hôpital Européen Georges Pompidou, 20-40 rue Leblanc, 75908 Paris cedex 15, France.

PMID: 14616731
DOI: 10.1046/j.1469-0691.2003.00764.x

Abstract

We describe a case of indolent vascular prosthesis infection due to Propionibacterium acnes. The microorganism was identified only by amplification and sequencing of 16S rDNA, while standard cultures remained negative. This observation underscores the usefulness of molecular techniques for the diagnosis of infection caused by fastidious microorganisms.

Publication types

Case Reports

MeSH terms

Anti-Bacterial Agents / therapeutic use
Blood Vessel Prosthesis / adverse effects*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Gram-Positive Bacterial Infections / drug therapy
Gram-Positive Bacterial Infections / microbiology*
Humans
Male
Middle Aged
Polymerase Chain Reaction
Propionibacterium acnes / genetics*
Propionibacterium acnes / growth & development
Prosthesis-Related Infections / drug therapy
Prosthesis-Related Infections / microbiology*
RNA, Ribosomal, 16S / chemistry
RNA, Ribosomal, 16S / genetics

Substances

Anti-Bacterial Agents
DNA, Bacterial
RNA, Ribosomal, 16S