[Establishment and characterization of a human squamous cell carcinoma cell line LK-52 which produce direct activator of coagulant factor X]

Hum Cell. 1992 Sep;5(3):287-91.
[Article in Japanese]

Abstract

Uterus origin squamous cell carcinoma cell line LK-52 was established from surgical specimen of lung metastatic nodule. LK-52 produce poorly differentiated squamous carcinoma in nude mouse, and doubling time in vitro was 38 hours. Chromosome analysis show various abnormality and main mode number was 67 and 68. LK-52 shed active procoagulant substance into culture medium. The culture medium of LK-52 shortening recalcification time of normal human plasma and factor VII or factor IX deficient plasma but not factor X deficient plasma. Procoagulant activity of LK-52 product may induced with direct activation of coagulant factor X. Procoagulant activity which produced by cancer cell may play a important roll in the unbalanced haemostasis of cancer patient.

Publication types

  • English Abstract

MeSH terms

  • Adult
  • Carcinoma, Squamous Cell / genetics
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cell Division
  • Cysteine Endopeptidases / metabolism*
  • Cysteine Endopeptidases / physiology
  • Female
  • Hemostasis
  • Humans
  • Karyotyping
  • Lung Neoplasms / genetics
  • Lung Neoplasms / metabolism*
  • Lung Neoplasms / pathology
  • Neoplasm Proteins*
  • Tumor Cells, Cultured*

Substances

  • Neoplasm Proteins
  • Cysteine Endopeptidases
  • cancer procoagulant