A microcantilever-based pathogen detector

Scanning. 2003 Nov-Dec;25(6):297-9. doi: 10.1002/sca.4950250605.

Abstract

The ability to detect small amounts of materials, especially bacterial organisms, is important for medical diagnostics and national security issues. Engineered micromechanical systems provide one approach for constructing multifunctional, highly sensitive, real-time, immunospecific biological detectors. We present qualitative detection of specific Salmonella enterica strains using a functionalized silicon nitride microcantilever. Detection is achieved due to a change in the surface stress on the cantilever surface in situ upon binding of a small number of bacteria. Scanning electron micrographs indicate that less than 25 adsorbed bacteria are required for detection.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies, Bacterial
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods
  • Microscopy, Atomic Force / instrumentation*
  • Microscopy, Atomic Force / methods*
  • Salmonella enterica / immunology
  • Salmonella enterica / isolation & purification*
  • Salmonella enterica / ultrastructure*
  • Silicon Compounds

Substances

  • Antibodies, Bacterial
  • Silicon Compounds
  • silicon nitride