Abstract
Collagen VI is a main extracellular matrix protein whose mutation is linked to myopathic diseases. In myoblasts and other cell types, collagen VI gene transcription peaks during cell-cycle exit that precedes differentiation, upon serum withdrawal or confluence. To get insight into this transcriptional regulation, we characterized a growth arrest responsive region (GARR) in the Col6a1 promoter responsible for this effect. In this work, we identify sterol regulatory element binding protein (SREBP) as a GARR binding protein and provide evidence that SREBP contributes to induction of Col6a1 transcription in serum free conditions. Furthermore, our data unveil a previously unexpected link between extracellular matrix production and LDL signaling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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CCAAT-Enhancer-Binding Proteins / physiology*
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Cell Nucleus / metabolism
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Collagen Type VI / biosynthesis*
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Collagen Type VI / genetics
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Culture Media, Serum-Free / pharmacology
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DNA, Complementary / metabolism
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DNA-Binding Proteins / physiology*
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Gene Expression Regulation
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Gene Library
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Glutathione Transferase / metabolism
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Lipoproteins, LDL / metabolism
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Mice
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Muscle Cells / metabolism
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NIH 3T3 Cells
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Plasmids / metabolism
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Promoter Regions, Genetic
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Protein Binding
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RNA / metabolism
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Sterol Regulatory Element Binding Protein 1
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Transcription Factors*
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Transcription, Genetic*
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Transcriptional Activation
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Two-Hybrid System Techniques
Substances
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CCAAT-Enhancer-Binding Proteins
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Collagen Type VI
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Culture Media, Serum-Free
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DNA, Complementary
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DNA-Binding Proteins
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Lipoproteins, LDL
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Srebf1 protein, mouse
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Sterol Regulatory Element Binding Protein 1
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Transcription Factors
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RNA
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Glutathione Transferase