Gene specific siRNA selector

Bioinformatics. 2004 Feb 12;20(3):430-2. doi: 10.1093/bioinformatics/btg437. Epub 2004 Jan 22.

Abstract

Small interfering RNA (siRNA) is used in functional genomics applications to decrease the expression of a target gene, which may yield a biological effect that suggests a function for the target gene. The siRNA design tool scans a target gene for candidate siRNA sequences that satisfy user-adjustable rules. Selected candidates are then screened to identify those siRNA sequences that are specific to the gene of interest.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms*
  • Computer-Aided Design*
  • Drug Design
  • RNA, Small Interfering / chemical synthesis
  • RNA, Small Interfering / chemistry*
  • RNA, Small Interfering / genetics*
  • Sensitivity and Specificity
  • Sequence Alignment / methods*
  • Sequence Analysis, RNA / methods*
  • Sequence Homology, Nucleic Acid
  • Software*

Substances

  • RNA, Small Interfering