Neurons arise in the basal neuroepithelium of the early mammalian telencephalon: a major site of neurogenesis

Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):3196-201. doi: 10.1073/pnas.0308600100. Epub 2004 Feb 12.

Abstract

Neurons of the mammalian CNS are thought to originate from progenitors dividing at the apical surface of the neuroepithelium. Here we use mouse embryos expressing GFP from the Tis21 locus, a gene expressed throughout the neural tube in most, if not all, neuron-generating progenitors, to specifically reveal the cell divisions that produce CNS neurons. In addition to the apical, asymmetric divisions of neuroepithelial (NE) cells that generate another NE cell and a neuron, we find, from the onset of neurogenesis, a second population of progenitors that divide in the basal region of the neuroepithelium and generate two neurons. Basal progenitors are most frequent in the telencephalon, where they outnumber the apically dividing neuron-generating NE cells. Our observations reconcile previous data on the origin and lineage of CNS neurons and show that basal, rather than apical, progenitors are the major source of the neurons of the mammalian neocortex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology
  • Animals
  • Cell Cycle Proteins / genetics
  • Crosses, Genetic
  • Embryonic and Fetal Development
  • Epithelial Cells / cytology
  • Female
  • Genes, Reporter
  • Genes, Tumor Suppressor
  • Green Fluorescent Proteins
  • Heterozygote
  • Immediate-Early Proteins / genetics
  • Luminescent Proteins / analysis
  • Luminescent Proteins / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Video
  • Mitosis
  • Neurons / cytology
  • Neurons / physiology*
  • Recombinant Fusion Proteins / analysis
  • Rhombencephalon / embryology
  • Rhombencephalon / growth & development
  • Telencephalon / embryology
  • Telencephalon / growth & development*
  • Tumor Suppressor Proteins

Substances

  • Btg2 protein, mouse
  • Cell Cycle Proteins
  • Immediate-Early Proteins
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Tumor Suppressor Proteins
  • Green Fluorescent Proteins