Abstract
We analyzed the TP53 and INK4A/ARF loci in 29 pediatric medulloblastomas. Mutually exclusive mutation in TP53, methylation of P14(ARF) or deletion of INK4A/ARF were identified in 21% (6/29) of tumors. Five of these alterations were detected in large cell/anaplastic medulloblastomas or tumors with significant anaplasia. Our data provide the first evidence that alterations within the TP53-ARF tumor suppressor pathway contribute to development of aggressive forms of medulloblastoma.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Anaplasia
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Carcinoma, Large Cell / metabolism*
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Cerebellar Neoplasms / metabolism*
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Child
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Cyclin-Dependent Kinase Inhibitor p16 / metabolism*
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DNA, Neoplasm / analysis
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Exons
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Gene Deletion
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Humans
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Medulloblastoma / metabolism*
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Methylation
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Mutation
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Polymerase Chain Reaction / methods
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sequence Analysis / methods
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Signal Transduction
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Tumor Suppressor Protein p14ARF
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Tumor Suppressor Protein p53 / metabolism*
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Tumor Suppressor Proteins
Substances
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Cyclin-Dependent Kinase Inhibitor p16
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DNA, Neoplasm
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RNA, Messenger
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Tumor Suppressor Protein p14ARF
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Tumor Suppressor Protein p53
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Tumor Suppressor Proteins