Secondary coronary artery vasospasm promotes cardiomyopathy progression

Am J Pathol. 2004 Mar;164(3):1063-71. doi: 10.1016/S0002-9440(10)63193-8.

Abstract

Genetic defects in the plasma membrane-associated sarcoglycan complex produce cardiomyopathy characterized by focal degeneration. The infarct-like pattern of cardiac degeneration has led to the hypothesis that coronary artery vasospasm underlies cardiomyopathy in this disorder. We evaluated the coronary vasculature of gamma-sarcoglycan mutant mice and found microvascular filling defects consistent with arterial vasospasm. However, the vascular smooth muscle sarcoglycan complex was intact in the coronary arteries of gamma-sarcoglycan hearts with perturbation of the sarcoglycan complex only within the adjacent myocytes. Thus, in this model, coronary artery vasospasm derives from a vascular smooth muscle-cell extrinsic process. To reduce this secondary vasospasm, we treated gamma-sarcoglycan-deficient mice with the calcium channel antagonist verapamil. Verapamil treatment eliminated evidence of vasospasm and ameliorated histological and functional evidence of cardiomyopathic progression. Echocardiography of verapamil-treated, gamma-sarcoglycan-null mice showed an improvement in left ventricular fractional shortening (44.3 +/- 13.3% treated versus 37.4 +/- 15.3% untreated), maximal velocity at the aortic outflow tract (114.9 +/- 27.9 cm/second versus 92.8 +/- 22.7 cm/second), and cardiac index (1.06 +/- 0.30 ml/minute/g versus 0.67 +/- 0.16 ml/minute/g, P < 0.05). These data indicate that secondary vasospasm contributes to the development of cardiomyopathy and is an important therapeutic target to limit cardiomyopathy progression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium Channel Blockers / pharmacology
  • Cardiomyopathies / drug therapy
  • Cardiomyopathies / etiology*
  • Cardiomyopathies / pathology*
  • Coronary Vasospasm / complications*
  • Coronary Vasospasm / drug therapy
  • Coronary Vasospasm / physiopathology*
  • Cytoskeletal Proteins / deficiency
  • Cytoskeletal Proteins / genetics
  • Disease Models, Animal
  • Disease Progression
  • Echocardiography
  • Fluorescent Antibody Technique
  • Heart / drug effects
  • Heart Function Tests / drug effects
  • Immunoblotting
  • Membrane Glycoproteins / deficiency
  • Membrane Glycoproteins / genetics
  • Mice
  • Mice, Mutant Strains
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / pathology
  • Myocardium / pathology
  • Sarcoglycans
  • Verapamil / pharmacology

Substances

  • Calcium Channel Blockers
  • Cytoskeletal Proteins
  • Membrane Glycoproteins
  • Sarcoglycans
  • Verapamil