Novel 473-bp deletion in XLRS1 gene in a Japanese family with X-linked juvenile retinoschisis

Graefes Arch Clin Exp Ophthalmol. 2004 Jul;242(7):561-5. doi: 10.1007/s00417-004-0878-y. Epub 2004 Feb 20.

Abstract

Purpose: To present the clinical features of two brothers with molecularly confirmed X-linked juvenile retinoschisis (xlRS) but with non-characteristic electrophysiological findings.

Methods: Comprehensive ophthalmological examinations were performed. The electroretinograms (ERGs) were recorded under ISCEV standards, and ERGs elicited by long-duration stimuli were also evaluated. Standard genetic analysis of peripheral blood leukocytes was performed.

Results: Molecular testing revealed a novel 473-bp deletion including exon 4 in the XLRS1 gene in both siblings. This resulted in a frameshift mutation and a premature termination at codon 78. The scotopic and photopic ERGs were reduced, but the "negative-type" ERG, characteristic of xlRS, was not observed. Flicker ERGs were also highly reduced. Long-duration stimuli elicited ERGs with a complete loss of the b-wave and a preservation of the off-response, i.e., negative-type ERG. The phenotype/genotype relationship was not determined.

Conclusion: The consistency of the ERGs elicited by long-duration stimuli in xlRS patients suggests that this type of stimuli provides responses that are a better indicator for the progression or stage of the disease.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Base Sequence
  • DNA Mutational Analysis
  • Electroretinography
  • Exons / genetics
  • Eye Proteins / genetics*
  • Frameshift Mutation*
  • Humans
  • Japan
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Retina / physiopathology
  • Retinoschisis / genetics*
  • Retinoschisis / physiopathology
  • Sequence Deletion / genetics*
  • Siblings

Substances

  • Eye Proteins
  • RS1 protein, human