Abstract
Crystal structures are reported for free and coenzyme A (CoA) bound forms of the YfdW protein from Escherichia coli, a representative type III CoA transferase. The structures reveal a two-domain protomer with interdomain connections forming a ring-like structure with a large central hole. Two protomers associate to form a highly intertwined dimer in which the hole of each ring is filled by the partner molecule. Each protomer binds a single CoA molecule and these CoA-binding sites are distant from one another in the dimer.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding Sites
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Coenzyme A / metabolism*
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Coenzyme A-Transferases / chemistry*
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Coenzyme A-Transferases / metabolism*
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Crystallography, X-Ray
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Dimerization
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Escherichia coli / enzymology*
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / metabolism*
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Protein Binding
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Protein Structure, Quaternary
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Protein Structure, Tertiary
Substances
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Escherichia coli Proteins
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Coenzyme A-Transferases
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Coenzyme A
Associated data
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PDB/1PQY
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PDB/1Q6Y
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PDB/1Q7E
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PDB/R1PQYSF
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PDB/R1Q6YSF
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PDB/R1Q7ESF