Production of hydroxyeicosatetraenoic acids and prostaglandins by a novel rat microglial cell line

J Neuroimmunol. 2004 Apr;149(1-2):130-41. doi: 10.1016/j.jneuroim.2003.12.022.

Abstract

We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Blotting, Western / methods
  • Cell Differentiation / drug effects
  • Cell Line
  • Chromatography, High Pressure Liquid / methods
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Glial Fibrillary Acidic Protein / metabolism
  • Hydroxyeicosatetraenoic Acids / metabolism*
  • Immunohistochemistry / methods
  • Lectins / metabolism
  • Macrophage Colony-Stimulating Factor / antagonists & inhibitors
  • Macrophage Colony-Stimulating Factor / pharmacology
  • Mass Spectrometry / methods
  • Microglia / metabolism*
  • Prostaglandin-Endoperoxide Synthases / metabolism
  • Prostaglandins / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Thymidine / pharmacokinetics
  • Time Factors
  • Tritium / pharmacokinetics
  • Tumor Necrosis Factor-alpha / analysis

Substances

  • Enzyme Inhibitors
  • Glial Fibrillary Acidic Protein
  • Hydroxyeicosatetraenoic Acids
  • Lectins
  • Prostaglandins
  • Tumor Necrosis Factor-alpha
  • Tritium
  • Macrophage Colony-Stimulating Factor
  • Prostaglandin-Endoperoxide Synthases
  • Thymidine