Abstract
We have established a clonal cell line derived from rat microglia that proliferates in response to macrophage-colony stimulating factor (CSF-1). Like primary neonatal microglia, these cells (named RTMGL1) exhibit a ramified morphology, bind isolectin B4, express CD68 and are weakly positive for CD11b and MHC class II. CSF-1-dependent proliferation requires intact signal transduction through several pathways. RTMGL1 synthesize multiple cyclooxygenase (COX) products including 11- and 15-hydroxyeicosatetraenoic acid (HETE) and express COX-2. RTMGL1 synthesize 5-HETE from arachidonic acid (AA) likely via a 5-lipoxygenase (LO). Thus, RTMGL1 have morphological and histological characteristics of primary microglia and metabolize AA via both COX and LO pathways.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Blotting, Western / methods
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Cell Differentiation / drug effects
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Cell Line
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Chromatography, High Pressure Liquid / methods
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Enzyme-Linked Immunosorbent Assay / methods
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Glial Fibrillary Acidic Protein / metabolism
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Hydroxyeicosatetraenoic Acids / metabolism*
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Immunohistochemistry / methods
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Lectins / metabolism
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Macrophage Colony-Stimulating Factor / antagonists & inhibitors
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Macrophage Colony-Stimulating Factor / pharmacology
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Mass Spectrometry / methods
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Microglia / metabolism*
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Prostaglandin-Endoperoxide Synthases / metabolism
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Prostaglandins / metabolism*
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Rats
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Rats, Sprague-Dawley
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Thymidine / pharmacokinetics
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Time Factors
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Tritium / pharmacokinetics
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Tumor Necrosis Factor-alpha / analysis
Substances
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Enzyme Inhibitors
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Glial Fibrillary Acidic Protein
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Hydroxyeicosatetraenoic Acids
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Lectins
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Prostaglandins
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Tumor Necrosis Factor-alpha
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Tritium
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Macrophage Colony-Stimulating Factor
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Prostaglandin-Endoperoxide Synthases
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Thymidine