Granularin, a novel molluscan opsonin comprising a single vWF type C domain is up-regulated during parasitation

FASEB J. 2004 May;18(7):845-7. doi: 10.1096/fj.03-0590fje. Epub 2004 Mar 19.

Abstract

Snails are intermediate hosts to schistosome parasites, some of which are the main cause of human schistosomiasis (bilharzia), and have been used as models for parasite-host interactions for a long time. Here, we have characterized a novel internal defense peptide of the snail Lymnaea stagnalis, of which the relative abundance in brain tissue increases upon infection with the avian schistosome Trichobilharzia ocellata. This protein, named granularin, is secreted by granular cells, which are numerous in the connective tissue surrounding the brain. The protein is unique because it comprises only a single Von Willebrand factor type C domain that is normally found in large transmembrane and secreted extracellular matrix proteins. The granularin gene is twice up-regulated during parasitation. Purified granularin stimulates phagocytosis of foreign particles by blood hemocytes. Together, our data indicate that granularin represents a novel protein that acts as an opsonin in the molluscan internal defense response.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA, Complementary / genetics
  • Disease Vectors
  • Ganglia, Invertebrate / metabolism
  • Gene Expression Regulation
  • Hemocytes / physiology
  • Host-Parasite Interactions / genetics*
  • Humans
  • Lymnaea / genetics
  • Lymnaea / immunology*
  • Lymnaea / metabolism
  • Lymnaea / parasitology
  • Molecular Sequence Data
  • Open Reading Frames
  • Opsonin Proteins / biosynthesis*
  • Opsonin Proteins / chemistry
  • Opsonin Proteins / genetics
  • Phagocytosis
  • Protein Structure, Tertiary
  • Schistosomatidae / immunology
  • Schistosomatidae / physiology*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • von Willebrand Factor / chemistry

Substances

  • DNA, Complementary
  • Opsonin Proteins
  • granularin protein, Lymnaea stagnalis
  • von Willebrand Factor

Associated data

  • GENBANK/AY530728