CCAAT/enhancer-binding protein-beta has a role in osteoblast proliferation and differentiation

Exp Cell Res. 2004 Apr 15;295(1):128-37. doi: 10.1016/j.yexcr.2004.01.004.

Abstract

CCAAT/enhancer binding protein beta (C/EBPbeta) is known to play an important role in the expression of several genes necessary for bone development and homeostasis including osteocalcin, IGF-1, and IL-6. In this study, we show that C/EBPbeta protein levels and, consequently, DNA-binding activity are temporally regulated, dramatically decreasing upon differentiation of MC3T3-E1 mouse osteoblasts. Corresponding with these results, the constitutive expression of C/EBPbeta LAP in MC3T3-E1 osteoblasts increased proliferation and suppressed osteogenic differentiation. Thus, C/EBPbeta LAP not only appears to participate in the regulation of genes associated with mature bone physiology, but is also a critical regulator of osteoblast growth and differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Animals
  • Animals, Newborn
  • CCAAT-Enhancer-Binding Protein-beta / genetics
  • CCAAT-Enhancer-Binding Protein-beta / physiology*
  • Cell Differentiation / physiology*
  • Cell Division / physiology*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation
  • Insulin-Like Growth Factor I / genetics
  • Interleukin-6 / genetics
  • Mice
  • Osteoblasts / cytology*
  • Osteocalcin / genetics

Substances

  • CCAAT-Enhancer-Binding Protein-beta
  • DNA-Binding Proteins
  • Interleukin-6
  • Osteocalcin
  • Insulin-Like Growth Factor I