Postprandial variations in fibrinolytic activity in middle-aged men are modulated by plasminogen activator inhibitor I 4G-675/5G genotype but not by the fat content of a meal

Am J Clin Nutr. 2004 Apr;79(4):577-81. doi: 10.1093/ajcn/79.4.577.

Abstract

Background: Decreased fibrinolytic activity is associated with an increased risk of ischemic heart disease and elevated plasma triacylglycerol concentrations.

Objective: The goal was to determine whether plasminogen activator inhibitor 1 (PAI-1) and fibrinolytic activities are influenced by 1) dietary fat intake from a test meal and 2) the PAI-1 4G allele.

Design: A parallel randomized controlled trial was used to compare the effect on fibrinolytic activity, measured as dilute clot lysis time, of high-oleate or high-palmitate test meals (both containing 50 g fat; both n = 18) with that of a low-fat test meal (15 g fat; n = 15) in men aged > 52 y. In a second study, postprandial changes in PAI-1 activity were measured in 32 men in response to a high-oleate meal containing 50 g fat. The results from both studies were analyzed according to PAI-1 4G-675/5G genotype.

Results: Fasting dilute clot lysis time was positively associated with body mass index (r = 0.326, P = 0.02) and was shortened postprandially (P < 0.00001) independent of the fat content of the meal. Fasting PAI-1 activity was higher in those carrying the 4G allele and was correlated with fasting plasma triacylglycerol concentrations (r = 0.48, P = 0.008) and factor VII coagulant activity (r = 0.46, P = 0.012) after adjustments for age, body mass index, and genotype. Plasma PAI-1 activity decreased significantly after a meal but was not associated with postprandial changes in plasma triacylglycerols after a high-fat meal. The postprandial increase in plasma triacylglycerols was higher in subjects carrying the 4G allele.

Conclusion: Fibrinolytic activity is not lower after meals rich in palmitate or oleate than after a low-fat meal.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cholesterol / blood
  • Dietary Fats / administration & dosage
  • Dietary Fats / pharmacology*
  • Fasting / metabolism
  • Fibrinolysis / drug effects*
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Oleic Acid / administration & dosage
  • Oleic Acid / pharmacology
  • Palmitic Acid / administration & dosage
  • Palmitic Acid / pharmacology
  • Plasminogen Activator Inhibitor 1 / genetics*
  • Plasminogen Activator Inhibitor 1 / metabolism
  • Postprandial Period

Substances

  • Dietary Fats
  • Plasminogen Activator Inhibitor 1
  • Oleic Acid
  • Palmitic Acid
  • Cholesterol