Abstract
Objective:
To evaluate the significance of somatic mutations of VHL gene and hypoxia-inducible factor-1alpha (HIF-1alpha) expression in primary renal clear cell carcinoma (RCC).
Methods:
Mutation of VHL gene and HIF-1alpha expression were detected by means of PCR, denaturing high-performance liquid chromatography (DHPLC), direct sequencing and immunohistochemistry in 32 samples from primary renal clear cell carcinoma patients.
Results:
In 32 RCC samples, 17 samples (53.1%) had and 32 samples of adjacent nonmalignant renal tissue had not mutations of VHL gene expression. Twelve RCC samples (70.6%) which had mutations of VHL gene expressed HIF-1alpha, and it had significant difference to 4 RCC (26.7%) samples which didn't have mutations of VHL gene (P < 0.05).
Conclusion:
Mutations of VHL gene may play a significant role in the tumorigenesis of RCC, and HIF-1alpha expression correlates with it.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma, Clear Cell / genetics*
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Adenocarcinoma, Clear Cell / pathology
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Adult
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Aged
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Carcinoma, Renal Cell / genetics*
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Carcinoma, Renal Cell / pathology
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Chromatography, Liquid
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit
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Immunohistochemistry
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Kidney / chemistry
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Kidney / metabolism
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Kidney / pathology
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Kidney Neoplasms / genetics*
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Kidney Neoplasms / pathology
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Male
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Middle Aged
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Mutation / genetics
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Polymerase Chain Reaction
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Transcription Factors / analysis
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Transcription Factors / genetics*
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Tumor Suppressor Proteins / analysis
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Tumor Suppressor Proteins / genetics*
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Ubiquitin-Protein Ligases / analysis
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Ubiquitin-Protein Ligases / genetics*
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Von Hippel-Lindau Tumor Suppressor Protein
Substances
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HIF1A protein, human
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Hypoxia-Inducible Factor 1, alpha Subunit
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Transcription Factors
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Tumor Suppressor Proteins
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Ubiquitin-Protein Ligases
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Von Hippel-Lindau Tumor Suppressor Protein
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VHL protein, human