Development of 99mTc labelled Lipiodol: biodistribution following injection into the hepatic artery of the healthy pig

Nucl Med Commun. 2004 Mar;25(3):291-7. doi: 10.1097/00006231-200403000-00013.

Abstract

Background: We develop a method for the radiolabelling of Lipiodol with Tc, using a lipophilic complex, [99mTc-(S2CPh)(S3Ph)2], dissolved in Lipiodol (99mTc-SSS Lipiodol).

Results: The labelling yield is high (96 +/- 0.8%), and the radiochemical purity satisfactory (92 +/- 2.6%). This labelling is reproducible and stable for up to 24 h in vitro. Studies carried out after injection into the hepatic artery of the healthy pig show that the biodistribution of 99mTc-SSS Lipiodol is comparable with that observed for 188Re Lipiodol.

Materials and methods: The 99mTc-SSS lipiodol was obtained after dissolving a chelating agent, previously labelled with 99mTc, in cold lipiodol. The radiochemical purity (RCP) of the labelling was checked immediately and at 24 h. The 99mTc-SSS lipiodol was injected into the hepatic artery of four healthy pigs for an ex-vivo biodistribution study. An autoradiographic study was performed in two cases.

Conclusions: Apart from the specific interest of a Lipiodol-bearing technetiated agent for carrying out dosimetric studies, the labelling of Lipiodol with 99mTc is a preliminary step towards the use of radiolabelling with the 188Re analogue.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Feasibility Studies
  • Hepatic Artery / drug effects
  • Hepatic Artery / metabolism*
  • Inactivation, Metabolic
  • Injections, Intra-Arterial
  • Iodized Oil / chemistry*
  • Iodized Oil / pharmacokinetics*
  • Isotope Labeling / methods*
  • Organ Specificity
  • Radiopharmaceuticals / chemical synthesis
  • Radiopharmaceuticals / pharmacokinetics
  • Swine
  • Technetium / chemistry*
  • Technetium / pharmacokinetics*
  • Tissue Distribution

Substances

  • Radiopharmaceuticals
  • Technetium
  • Iodized Oil