Differential expansion of umbilical cord blood mononuclear cell-derived natural killer cells dependent on the dose of interleukin-15 with Flt3L

Exp Hematol. 2004 Feb;32(2):202-9. doi: 10.1016/j.exphem.2003.10.013.

Abstract

Objective: We investigated the effect of interleukin-15 (IL-15) with Flt3 ligand (Flt3L) on the expansion and activation of NK cells derived from umbilical cord blood mononuclear cells (UCB-MNCs).

Materials and methods: UCB-MNCs were cultured at 1 to 100 ng/mL of IL-15 + Flt3L (10 ng/mL) compared with 1 to 500 ng/mL of IL-2 + Flt3L (10 ng/mL). Cultured cells were assessed for surface marker and we calculated absolute number of NK cells and T cells. The cytotoxic activity was analyzed with purified NK cells.

Results: After 2 weeks culture with 5 ng/mL of IL-15 + Flt3L, the fold inductions of absolute number of NK cells significantly increased to 20.9-fold +/- 9.3-fold of the number of NK cells on day 0 (p < 0.05), with 24.4-fold +/- 16.1-fold of T cells. But with 50 ng/mL of IL-15 + Flt3L, fold induction of NK cells decreased to 5.1-fold +/- 3.9-fold, while T cells showed 34.8-fold +/- 18.7-fold (n = 8). The proportion of NK vs T cells showed to be significantly higher (1.61 +/- 0.91) with 5 ng/mL of IL-15 than with 50 ng/mL of IL-15 (0.12 +/- 0.03). Such proportional change of NK/T cells could not be observed with IL-2. Immunophenotypes of CD56, CD16, LFA1, CD94, CD8, and perforin of cultured NK cells with 10 ng/mL of IL-15 + Flt3L showed the same pattern of those with 50 ng/mL of IL-2 + Flt3L. Cytotoxic activity against K562 of cultured NK cells resulted in the same level as adult peripheral blood (PB)-derived NK cells.

Conclusions: Higher induction of NK cells derived from UCB-MNCs was achieved by low dose (5 to 10 ng/mL) rather than high dose (> 50 ng/mL) of IL-15.

MeSH terms

  • CD56 Antigen / analysis
  • Cells, Cultured
  • Cytotoxicity, Immunologic
  • Fetal Blood / cytology*
  • Humans
  • Immunophenotyping
  • Interleukin-15 / pharmacology*
  • Interleukin-2 / pharmacology
  • Killer Cells, Natural / cytology
  • Killer Cells, Natural / drug effects*
  • Killer Cells, Natural / immunology
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects*
  • Membrane Proteins / pharmacology*

Substances

  • CD56 Antigen
  • Interleukin-15
  • Interleukin-2
  • Membrane Proteins
  • flt3 ligand protein