Genetic and biochemical characterization of a chromosome-encoded carbapenem-hydrolyzing ambler class D beta-lactamase from Shewanella algae

Antimicrob Agents Chemother. 2004 May;48(5):1670-5. doi: 10.1128/AAC.48.5.1670-1675.2004.

Abstract

A chromosome-encoded beta-lactamase gene from Shewanella algae clinical isolate KB-1 was cloned and expressed in Escherichia coli. It encoded the Ambler class D enzyme OXA-55, sharing less than 55% identity with any other oxacillinases. Although conferring a narrow-spectrum beta-lactam resistance phenotype, OXA-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed in S. algae KB-1. Very similar oxacillinases were found in other S. algae isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Base Sequence
  • Carbapenems / metabolism*
  • Child
  • Chromosomes, Bacterial / genetics
  • Cloning, Molecular
  • Conjugation, Genetic
  • DNA, Bacterial / biosynthesis
  • DNA, Bacterial / genetics
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology
  • Female
  • Genes, Bacterial
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Isoelectric Focusing
  • Kinetics
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Penicillinase / metabolism
  • Phenotype
  • Plasmids
  • Shewanella / enzymology*
  • Shewanella / genetics*
  • beta-Lactamases / genetics*
  • beta-Lactamases / isolation & purification
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • Carbapenems
  • DNA, Bacterial
  • Penicillinase
  • ambler class D beta-lactamase, S oneidensis
  • beta-Lactamases