Abstract
Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Antibodies, Bacterial
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Bacterial Proteins / genetics*
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Bacterial Proteins / immunology
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Base Sequence
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Gene Expression
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Genes, Bacterial*
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Helicobacter pylori / genetics*
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Helicobacter pylori / immunology
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Helicobacter pylori / pathogenicity
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Humans
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Immunoblotting / methods
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Immunoblotting / statistics & numerical data
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Molecular Sequence Data
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Polymerase Chain Reaction
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sensitivity and Specificity
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Virulence / genetics
Substances
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Antibodies, Bacterial
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Bacterial Proteins
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DNA Primers
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DNA, Bacterial
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Recombinant Proteins