Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes

J Clin Microbiol. 2004 May;42(5):2294-7. doi: 10.1128/JCM.42.5.2294-2297.2004.

Abstract

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Protozoan / genetics
  • Genes, Protozoan*
  • HSP70 Heat-Shock Proteins / genetics*
  • Humans
  • Leishmania / classification*
  • Leishmania / genetics*
  • Leishmania / isolation & purification
  • Leishmaniasis / parasitology
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • Polymorphism, Restriction Fragment Length
  • Protozoan Proteins / genetics*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • DNA, Protozoan
  • HSP70 Heat-Shock Proteins
  • Protozoan Proteins