Molecular characterisation of SENV and TTV infections in hepatopathic liver-transplant patients. Brief Report

Arch Virol. 2004 Jul;149(7):1423-33. doi: 10.1007/s00705-004-0320-0. Epub 2004 Apr 5.

Abstract

The presence of SENV and TTV infections among 50 patients who had undergone liver transplantation was evaluated. UTR amplification showed that 46 (92%) sera were positive. ORF-1 amplification showed that 25 (50%) patients were positive for either SENV (51.3%), TTV (10.8%), or both (37.8%) all confirmed by sequencing and phylogenetic analysis. SENV-D and SENV-H were the most prevalent viruses. The phylogenetic analysis of isolates showed that whereas SENV-D and SENV-G viruses showed sequence stability and strain persistence, SENV-H had cleared or mutated. Biological differences seem to exist among different genotypes in terms of viral replication and their persistence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 5' Untranslated Regions
  • Circoviridae / classification
  • Circoviridae / genetics*
  • Circoviridae / isolation & purification*
  • Circoviridae Infections / virology*
  • DNA, Viral / blood
  • DNA, Viral / chemistry
  • Female
  • Genes, Viral
  • Genotype
  • Humans
  • Liver Transplantation*
  • Male
  • Middle Aged
  • Open Reading Frames
  • Phylogeny
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Torque teno virus / classification
  • Torque teno virus / genetics*
  • Torque teno virus / isolation & purification

Substances

  • 5' Untranslated Regions
  • DNA, Viral