Mouse allergen exposure and immunologic responses: IgE-mediated mouse sensitization and mouse specific IgG and IgG4 levels

Ann Allergy Asthma Immunol. 2004 Aug;93(2):171-8. doi: 10.1016/S1081-1206(10)61471-8.

Abstract

Background: Although there is evidence that contact with mice is associated with IgE-mediated mouse sensitization and mouse specific antibody responses, the exposure-response relationships remain unclear.

Objective: To determine whether IgE-mediated mouse sensitization and mouse specific IgG (mIgG) and mIgG4 levels increase with increasing Mus m 1 exposure.

Methods: One hundred fifty-one workers at a mouse research and production facility were studied. Exposure assignments were made by linking participants to airborne Mus m 1 concentrations in their respective work areas. Cumulative exposure was estimated by multiplying airborne Mus m 1 concentration by duration of employment. Serum mIgG and mIgG4 levels were quantified by antigen-binding assays, and IgE-mediated mouse sensitization was evaluated by skin prick testing (SPT).

Results: Prevalence rates of mouse SPT sensitivity and of high levels of mIgG and mIgG4 were increasingly higher by quintiles of increasing cumulative exposure (P < .01 for SPT, mIgG, and mIgG4). After adjusting for age, sex, and atopy, the log odds ratio (OR) of having positive mouse SPT results was linearly related to cumulative exposure (r2 = 0.87), as was the log OR of having a high mIgG level (r2 = 0.86). Quintile of cumulative exposure was an independent predictor of both SPT sensitivity (OR, 1.7; 95% confidence interval, 1.2-2.5) and a high mIgG level (OR, 1.7; 95% confidence interval, 1.2-2.4).

Conclusions: IgE-mediated mouse sensitization and mIgG and mIgG4 levels were related to cumulative exposure in a dose-dependent manner. Thus, strategies to prevent allergy to mice should remain focused on reducing mouse allergen exposure.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Air Pollutants, Occupational / adverse effects
  • Air Pollutants, Occupational / immunology
  • Allergens / administration & dosage
  • Allergens / adverse effects*
  • Allergens / immunology*
  • Animals
  • Antibody Specificity / immunology
  • Antigens, Dermatophagoides / administration & dosage
  • Antigens, Dermatophagoides / adverse effects
  • Antigens, Dermatophagoides / immunology
  • Biomarkers / blood
  • Cross-Sectional Studies
  • Dose-Response Relationship, Immunologic
  • Female
  • Humans
  • Hypersensitivity, Immediate / epidemiology
  • Hypersensitivity, Immediate / etiology*
  • Hypersensitivity, Immediate / therapy
  • Immunization
  • Immunoglobulin G / immunology
  • Immunoglobulin G / metabolism
  • Maine / epidemiology
  • Male
  • Medical Laboratory Personnel
  • Mice
  • Multivariate Analysis
  • Occupational Exposure / adverse effects*
  • Occupational Exposure / analysis
  • Prevalence
  • Radioallergosorbent Test
  • Respiratory Hypersensitivity / epidemiology
  • Respiratory Hypersensitivity / etiology
  • Respiratory Hypersensitivity / therapy
  • Risk Factors
  • Sensitivity and Specificity
  • Skin Tests
  • Statistics as Topic

Substances

  • Air Pollutants, Occupational
  • Allergens
  • Antigens, Dermatophagoides
  • Biomarkers
  • Immunoglobulin G